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Fig. 6 | BMC Biomedical Engineering

Fig. 6

From: Spiropyran as a potential molecular diagnostic tool for double-stranded RNA detection

Fig. 6

MC can be used to detect cellular dsRNAs isolated from HeLa cells. a The absorbance spectra of MC when 350 μM of various RNA samples (synthetic and cellular) were added. b Fragmentation of HeLa rRNAs by RNase T1 (lane 3) or RNase A (lane 4) was confirmed using gel electrophoresis. Lane 1 shows the control sample without any RNases and lane 2 shows the control RNAs after incubating it for 30 min in TDW. c The effect of RNase treatment on MC absorbance spectra. d Quantification of the absorbance spectra upon addition of RNase T1 or RNase A treated HeLa RNAs to MC. Average of three biological replicates is shown with error bars indicating s.e.m. Statistical tests show that both RNase T1 and RNase A treated samples are significantly different from each other and from the control sample

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