Table 1 MC-dsRNA binding constants. Macroscopic binding constants in 9 mM Na + ions and 1 mM cacodylate pH 7 (buffer 1) and for nuclease free TDW at pH 7 (buffer 2)
From: Spiropyran as a potential molecular diagnostic tool for double-stranded RNA detection
ds-RNA | Bmax (Δ%) | K (M−1) | Bmax (Δ%) | K (M−1) |
|---|---|---|---|---|
Buffer 1 | Buffer 2 | |||
Poly GC | 25.1 | 7352 | 90.6 | 11,111 |
Poly AU | 14.2 | 3891 | 52.5 | 7042 |
50% GC content dsRNA | ~ 20 | ~ 5000 | ~ 70 | ~ 8620 |