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Table 2 The pKa values and micro/macroscopic binding constants for the MC-dsRNA interaction. The pKa values and micro/macroscopic binding constants calculated in the experiments. From these values, the molar ratio of the binding species for MCH+ to MC was observed to be 90:10. The schematic shows the binding and protonation occurring due to interaction with dsRNAs

From: Spiropyran as a potential molecular diagnostic tool for double-stranded RNA detection

Parameter

Value

Schematic

pKaMC

3.8

pKaMC-RNA

7.7

Kmacroscopic

8.62 × 103

Kmicroscopic

1.13 × 104

MCH*RNA:MC*RNA at pH 7

~ 90:10