Fig. 2
Effects of TGF-β1 stimulation in combination with macromolecular crowding on IPF fibroblast nuclear integrity in vitro. a Immunocytochemical images of Hoechst stained IPF fibroblast nuclei after exposure to increasing concentrations of TGF-β1 for 72 h. b Quantification of cell number identified in (A) using Hoechst-stained nuclei and computational algorithm to define nuclei area. c & d Images and quantification of comparison of cell number at time zero (T0), and after 72 h TGF-β1 (1 ng/ml) in the presence of Ficoll media. Quantification is represented as % cell count in comparison to the number of cells at the start of the experiment (T0). Cell counts were quantified from two fields of view at 10x magnification. Scale bars represent 200 μm. Images are representative of at least 3 independent tests. Data points represent mean ± SEM