Skip to main content

Advertisement

Table 1 ‘Scar-in-a-jar’ HCS assay metrics and compound potency for standard inhibitors

From: A high content, phenotypic ‘scar-in-a-jar’ assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts

  pIC50 Assay metrics
MEAN STDEV %CV Assay window n Z prime
PGE2 7.65 0.98 12.7 4.64 8 0.50
SB-525334 6.79 0.33 4.8 4.63 28 0.49
CZ415 6.38 0.31 4.8 4.67 25 0.51
  1. Average pIC50, standard deviation (STDEV), and % coefficient of variance (%CV) values of the assay controls: SB-525334, PGE2 and CZ415 and assay parameters to measure assay performance and robustness