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Fig. 2 | BMC Biomedical Engineering

Fig. 2

From: Osteogenic potential of heterogeneous and CD271-enriched mesenchymal stromal cells cultured on apatite-wollastonite 3D scaffolds

Fig. 2

Characteristics of PA-MSC and CD271-MSC in 2D culture. Basic characteristics of paired PA-MSCs and CD271-MSCs were assessed during 2D in vitro expansion. a The cumulative population doublings of paired PA-MSC and CD271-MSC. b, c Representative phase contrast images showing the fibroblast-like morphology of PA-MSC (top row) and CD271-MSC (bottom row) respectively. Scale bars indicate 200 μm. d, e Representative images showing tri-lineage differentiation of PA-MSC and CD271-MSC respectively. From left to right: adipogenic differentiation (oil-red-O staining lipid vacuoles), osteogenic differentiation (ALP stain in blue and mineralization in black), chondrogenic differentiation (alcian blue staining glycosaminoglycans). Scale bars on all images indicate 200 μm. All images were taken on a NIKON spinning disk microscope. f, g Graphs showing the representative histogram and the percentage of positive cells for the phenotypic markers of MSCs. Error bars represent the SEM of 3 independent experiments

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BMC Biomedical Engineering

ISSN: 2524-4426

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