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Fig. 1 | BMC Biomedical Engineering

Fig. 1

From: Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro

Fig. 1

a Procedure of renal organoid production. C indicates CHIR-99021 and F indicates FGF9. The detailed protocol is described in the Methods section. b Macroscopic image. c OCT section image of organoids at 12 days after 3D formation. d Phase contrast microscopic image of a renal organoid e) immunostaining with E-cadherin (red fluorescence) and FITC-LET lectin (green fluorescence), or f) CK8 (red fluorescence) and PAX2 (green fluorescence) and g) WT1 (green fluorescence). Blue fluorescence indicates the counterstained cell nuclei (e–g). CDH1 (h) and NPHS1 (i) gene expression after 3D formation of renal organoid induction is indicated as graphs

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BMC Biomedical Engineering

ISSN: 2524-4426

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