Fig. 2
Proliferation and migration of endothelial cells in response to DS, ES, SS secretomes. a Proliferation: endothelial cell culture medium was supplemented with bFGF (10 ng/ml) and VEGF (10 ng/ml) or AD, DS, ES, SS secretome (5 and 10%) and 3H uptake determined 72 h later. b Scratch assay: representative pictures of human dermal endothelial cells cultured in the presence of 10% AD secretome or 10% SS secretome at 0 and 24 h. c Scratch assay: migration of endothelial cells into the scratch area in response to bFGF (10 ng/ml) or AD, DS, ES and SS secretome (1 and 10%). Area covered (mm2) by migrated endothelial cells is shown. C = unsupplemented endothelial cell cultures; significance was determined using a repeated measures one-way ANOVA followed by a Dunnett’s multiple comparisons test. *P < 0.05, **P < 0.01. Data is shown for 4–5 donors as mean ± SEM. cpm = counts per minute