TY - JOUR AU - Jaimes, Rafael AU - McCullough, Damon AU - Siegel, Bryan AU - Swift, Luther AU - Hiebert, James AU - McInerney, Daniel AU - Posnack, Nikki Gillum PY - 2019 DA - 2019/09/27 TI - Lights, camera, path splitter: a new approach for truly simultaneous dual optical mapping of the heart with a single camera JO - BMC Biomedical Engineering SP - 25 VL - 1 IS - 1 AB - Optical mapping of transmembrane voltage and intracellular calcium is a powerful tool for investigating cardiac physiology and pathophysiology. However, simultaneous dual mapping of two fluorescent probes remains technically challenging. We introduce a novel, easy-to-use approach that requires a path splitter, single camera and excitation light to simultaneously acquire voltage and calcium signals from whole heart preparations, which can be applied to other physiological models – including neurons and isolated cardiomyocytes. SN - 2524-4426 UR - https://doi.org/10.1186/s42490-019-0024-x DO - 10.1186/s42490-019-0024-x ID - Jaimes2019 ER -