Table 1 Example of optical mapping configurations using dual sensors

Sensor

^aAndor Zyla 4.2 PLUS

Photometrics EMCCD

SciMedia N256

IDS UI-3220CP-M-GL

IDS UI-3360CP-NIR-GL

Peak QE

82%

> 92%

55%

50%

60%

Sensor size (MM)

1.7 × 2.5

3.1 × 3.1

17.6 × 17.6

2.9 × 4.5

6 × 11.3

Pixel size (UM)

6.5

24

69

6

5.5

No. of pixels

256 × 384 (rat) 640 × 512 (pig)

128 × 128

256 × 256

120 × 160

160 × 220

Digitization

16-bit

16-bit

14-bit

10-bit

12-bit

Effective frame rate

814 (rat) 406 (pig)

530

1000

400 (pig)

1000

Total cost, # cameras

$18 k, 1 camera

$70 k, 2 cameras

$107 k, 2 cameras

$1600, 2 cameras

$2400, 2 cameras