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Table 2 Comparison of novel single sensor optical mapping system to dual sensor configurations

From: Lights, camera, path splitter: a new approach for truly simultaneous dual optical mapping of the heart with a single camera

Reference Jaimes et al. (2019)a Lee et al. (2011) Bachtel et al. (2011) Yamanaka et al. (2012) Scull et al. (2012)
Dyes used Rhod2 and RH237 Fura-2 and Di-4-ANBDQPQ Di-4-ANEPPS Rhod2 and RH237 X-Rhod-1 and Di-4-ANEPPS
Biological sample Isolated heart Isolated heart Isolated heart Isolated heart Cell monolayer
No. of pixels 256 × 384 (rat) 640 × 512 (pig) 64 × 64 128 × 64 512 × 512 504 bundled optical fibers and PDA
Effective frame rate per channel 814 (rat) 406 (pig) 465 (variable) 375 125 488.3
Signal-to-noise ~ 12 50–200 5.8 NA 21
Necessary hardware Single, constant wavelength light-source. Path splitter required Four wavelength LEDs with custom-built microcontroller Blue and cyan LEDs mounted on PCBs, custom hardware and software for rapid switching LED ring light consisting of blue and yellow LEDs Dual wavelength LED matrix, custom passband filter, A/D triggering board, custom LabVIEW program
Key differences Simultaneous imaging of Vm and Ca, off-the-shelf components Interleaved, Ratiometric Vm and ratiometric Ca Sequential imaging, Ratiometric Vm measurements only Sequential imaging, Emission filter wheel, allows for more possible dye combinations Sequential imaging, Crosstalk reduction algorithm
  1. anote: novel configuration described in this manuscript; calculated signal-to-noise ratio measurements are listed in Additional file 5: Table S2