Fig. 5

Osteogenic potential of A-W scaffold seeded PA-MSC and CD271-MSC. Scanning electron microscopy images (a-d) highlight areas of matrix deposition (*) and nodule formations (<) on MSC seeded scaffold after 14 days of culture in osteogenic induction medium. Scale bars represent 2 mm (a, c) and 50 μm (b, d). Images are representative of 3 independent experiments. Quantification of osteogenesis of paired MSCs is shown through ALP activity normalised to the DNA content (e). Error bars represent the SEM of 3 independent experiments. The osteogenic potential of non-cultured BM-MNCs seeded on A-W scaffolds, with or without CD271-enrichment, was presented as DNA quantification (f) and ALP activity (g). Error bars represent the SEM of 3 independent experiments. *p = 0.026 (paired t-test)