Fig. 7
Cytocompatibility of pristine EDL and EDL:Ag nanocomposites. Fluorescent images of primary ventral mesencephalic mixed cell population cultured for 3, 7 and 14 days on each of the experimental and control groups: bare Pt, pristine EDL and EDL:Ag 10:1 composite; neurons are visualized by anti β-tubulin III (red), astrocyte cells by anti-GFAP (green) and nuclei by DAPI (blue); the scale bar represents 20 μm (a). Cell density (%) analysis of astrocyte and neuron presence on each of the experimental and control groups (b) and neural length analysis of the experimental and control groups (c); Calcium imaging and substrate stimulation for a bare, EDL-coated and EDL:Ag 10:1-coated ITO electrode: fluorescence tracings show the appearance of Ca2+ episodes; short lines highlight the stimulation pulses (d); 
= p < 0.05, N = 3