Fig. 4

Live brain organoid imaging. a Neuronal progenitor behaviour in brain organoids (see also Supplementary Movie 2). i. Whole brain organoid projection. ii. Magnified view of a brain organoid cortical area. iii. Neuronal movement tracking in a brain organoid region and still images showing INM. iv. Examples of neuronal progenitor oblique and horizontal cell division. Scale bars are 500 μm (i) and 50 μm (ii-iv). b Visualisation of GSC tumour growth and invasion within healthy brain organoids using confocal microscopy (see also Supplementary Movie 3). i. Time-lapse (h) of cerebral organoids injected with patient-derived glioma stem cells (PD-GSC). ii. Magnified view of region highlighted in i (left) with individual cell trajectories (right). iii. Still images showing GSC movement within the brain organoid. In a and b, Panels were made with cropped images (same size for all time points and conditions). Image’s intensity levels were contracted (same extent for all time points across all conditions but different across different channels) from their original 16-bit range to 8-bit RGB for Figure preparation in Adobe Illustrator